LoC sensor array platform for real-time coagulation measurements

Abstract

This paper reports a MEMS-based sensor array enabling multiple clot-time tests in one disposable microfluidic cartridge using plasma. The versatile LoC (Lab-on-Chip) platform technology is demonstrated here for real-time coagulation tests (activated Partial Thrompoblastin Time (aPTT) and Prothrombin Time (PT)). The system has a reader unit and a disposable cartridge. The reader has no electrical connections to the cartridge, which consists of multiple microfluidic channels and MEMS microcantilevers placed in each channel. Microcantilevers are made of electro-plated nickel and actuated remotely using an external electro-coil. The read-out is also conducted remotely by a laser and the phase of the MEMS oscillator is monitored real-time. The system is capable of monitoring coagulation time with a precision estimated at 0.1sec.