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dc.contributor.authorWang, S.
dc.contributor.authorTasoglu, S.
dc.contributor.authorChen, P. Z.
dc.contributor.authorChen, M.
dc.contributor.authorAkbaş, Ragıp
dc.contributor.authorWach, S.
dc.contributor.authorOzdemir, C. İ.
dc.contributor.authorGurkan, U. A.
dc.contributor.authorGiguel, F. F.
dc.contributor.authorKuritzkes, D. R.
dc.contributor.authorDemirci, U.
dc.date.accessioned2014-04-10T11:37:15Z
dc.date.available2014-04-10T11:37:15Z
dc.date.issued2014-01-22
dc.identifier.issn2045-2322
dc.identifier.urihttp://hdl.handle.net/10679/324
dc.identifier.urihttp://www.nature.com/srep/2014/140121/srep03796/full/srep03796.html
dc.description.abstractHIV has become one of the most devastating pathogens in human history. Despite fast progress in HIV-related basic research, antiretroviral therapy (ART) remains the most effective method to save AIDS patients' lives. Unfortunately, ART cannot be universally accessed, especially in developing countries, due to the lack of effective treatment monitoring diagnostics. Here, we present an inexpensive, rapid and portable micro-a-fluidic platform, which can streamline the process of an enzyme-linked immunosorbent assay (ELISA) in a fully automated manner for CD4 cell count. The micro-a-fluidic CD4 cell count is achieved by eliminating operational fluid flow via “moving the substrate”, as opposed to “flowing liquid” in traditional ELISA or microfluidic methods. This is the first demonstration of capturing and detecting cells from unprocessed whole blood using the enzyme-linked immunosorbent assay (ELISA) in a microfluidic channel. Combined with cell phone imaging, the presented micro-a-fluidic ELISA platform holds great promise for offering rapid CD4 cell count to scale up much needed ART in resource-constrained settings. The developed system can be extended to multiple areas for ELISA-related assays.en_US
dc.description.sponsorshipthe Center for Integration of Medicine and Innovative Technology ; the U.S. Army Medical Research & Materiel Command (USAMRMC) ; the Telemedicine & Advanced Technology Research Center (TATRC).
dc.language.isoengen_US
dc.publisherNatureen_US
dc.relation.ispartofScientific Reports
dc.rightsopenAccess
dc.titleMicro-a-fluidics ELISA for rapid CD4 cell count at the point-of-careen_US
dc.typeArticleen_US
dc.description.versionpublisher version
dc.peerreviewedyesen_US
dc.publicationstatuspublisheden_US
dc.contributor.departmentÖzyeğin University
dc.contributor.authorID181923
dc.contributor.ozuauthorAkbaş, Ragıp
dc.identifier.volume4
dc.identifier.wosWOS:000330044300001
dc.identifier.doi10.1038/srep03796
dc.subject.keywordsTranslational researchen_US
dc.subject.keywordsLaboratory techniques and proceduresen_US
dc.identifier.scopusSCOPUS:2-s2.0-84892984932
dc.contributor.authorMale1


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