Acarturk, B. C.Sandalcı, IlgınHull, N. M.Bundur, Zeynep BaşaranBurris, L. E.2023-08-152023-08-152023-080958-9465http://hdl.handle.net/10679/8680https://doi.org/10.1016/j.cemconcomp.2023.105115Creation of more durable concrete is one pathway to achieving improved sustainability and carbon footprint over a concrete structure's life. Microbially induced calcite precipitation has been shown to densify concrete microstructure and fill cracks, reducing moisture transport. One challenge associated with the longevity of bacterial-concrete systems is the high pH environment of the cement paste. Herein, two approaches to address this challenge were investigated: (i) sustainable binder systems, such as calcium sulfoaluminate (CSA) cement and fly ash substitutions of ordinary portland cement (OPC), which lead to lower pH systems, and (ii) non-axenic bacterial cultures, which may facilitate growth of more alkaline-resistant bacteria. Mechanical properties, water absorption, self-healing ability, and survivability of the bacterial systems were tracked, finding that incorporation of non-axenic bacteria did not result in increased bacteria survivability compared to axenic bacteria. However, both bacteria healed cracks [removed]engrestrictedAccessarticle14100100124900000110.1016/j.cemconcomp.2023.105115Bacteria viabilityCalcium sulfoaluminate cementFly ashSelf-healing of cracksSustainability2-s2.0-85163856043